Role of specimen collection in preanalytical variation of metalloproteinases and their inhibitors in blood.

نویسندگان

  • K Jung
  • L Nowak
  • M Lein
  • W Henke
  • D Schnorr
  • S A Loening
چکیده

bound iron. We now use the Unimate method as our routine method. With this method, serum iron is determined linearly up to 180 jmol1L and the detection limit is 0.9 j.tmol/L. Withinrun CVs are 0.4% (iron concentration, 7.7-38.0 j.tmoVL) and the analytical recoveries of added iron are 99.2-101.2%. We think that this colorimetric method, which does not involve sample deproteinization or heating, would be an ideal reference method for measuring serum iron.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Evaluation of Error Types and Turnaround Time of Preanalytical Phase in Biochemistry and Hematology Laboratories

Background& Objective: Each laboratory should determine the type of errors and turnaround time (TAT), especially in the preanalytical phase to report quality and timeliness of the test results. The current study aimed at investigating the common causes of preanalytical errors in biochemistry and hematology laboratories and evaluating the preanalytical ...

متن کامل

Changing Roles of Matrix Metalloproteases and Their Inhibitors, TIMPs, During Tumor Progression and Angiogenesis

Inhibition of matrix-metalloproteinases (MMPs) by tissue inhibitors of metalloproteinases (TIMPs) has been shown in vivo to decrease metastasis and tumor-associated angiogenesis. Our laboratory is interested in understanding the role of these proteins at the pericellular microenvironment of tumor and endothelial cells. Secretion of MMPs by tumor cells enables the migration, invasion and metasta...

متن کامل

P 88: Matrix Metalloproteinases in Neuroinflammation

Matrix metalloproteinases (MMPs) are a family of neutral proteinases that are important in normal development, cellular differentiation or migration, angiogenesis, neurogenesis, wound repair, and a wide range of pathological processes such as oxidative stress and neuroinflammation. MMPs have been demonstrated to increase the permeability of the blood–brain barrier (BBB) by degrading the c...

متن کامل

Expression of Matrix Metalloproteinase-2/9 and Tissue Inhibitor of Metalloproteinase-1/2 as Predictive Factors in Oropharyngeal Squamous Cell Carcinoma

Introduction: Metalloproteinases and their tissue inhibitors play an important role in the metastases formation. A multistage process of carcinogenesis requires the involvement of numerous enzymes and compounds that facilitate the expansion of tumor cells. The formation of metastases depends on both metalloproteinases and tissue inhibitors activation leading to the acti...

متن کامل

Effects of pre-analytical processes on blood samples used in metabolomics studies

Every day, analytical and bio-analytical chemists make sustained efforts to improve the sensitivity, specificity, robustness, and reproducibility of their methods. Especially in targeted and non-targeted profiling approaches, including metabolomics analysis, these objectives are not easy to achieve; however, robust and reproducible measurements and low coefficients of variation (CV) are crucial...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Clinical chemistry

دوره 42 12  شماره 

صفحات  -

تاریخ انتشار 1996